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. 2017 Aug 7;6(1):A0060. doi: 10.5702/massspectrometry.A0060

Fig. 10. Negative-ion MALDI mass spectra of SALSA-derivatized A2-type N-glycans released from SGP standards; (a) α2,3-SGP and (b) α2,6-SGP. Mass spectra were obtained by MALDI-QIT-TOF MS in the negative-ion mode after on-plate 3-AQ labeling using 3-AQ/CA liquid matrix. Associated dehydrated peaks correspond to laser-induced dehydration between sulfate anions and N-glycans. The 100% stacked column chart (c) shows relative intensities of the correctly-amidated forms (black) and their singly-misconverted forms (gray). Doubly-misconverted forms were not detected in any cases. Error bars indicate standard deviations of quadruple analysis. Reproduced with permission from ref. 72. Copyright (2017) American Chemical Society.

Fig. 10. Negative-ion MALDI mass spectra of SALSA-derivatized A2-type N-glycans released from SGP standards; (a) α2,3-SGP and (b) α2,6-SGP. Mass spectra were obtained by MALDI-QIT-TOF MS in the negative-ion mode after on-plate 3-AQ labeling using 3-AQ/CA liquid matrix. Associated dehydrated peaks correspond to laser-induced dehydration between sulfate anions and N-glycans. The 100% stacked column chart (c) shows relative intensities of the correctly-amidated forms (black) and their singly-misconverted forms (gray). Doubly-misconverted forms were not detected in any cases. Error bars indicate standard deviations of quadruple analysis. Reproduced with permission from ref. 72. Copyright (2017) American Chemical Society.