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. 2017 Aug 7;6(1):A0060. doi: 10.5702/massspectrometry.A0060

Fig. 4. Representative negative-ion CID spectra of desialylated N-glycans derived from the HER2 protein (SK-BR-2 cell line). CID spectra of (a) Hex7HexNAc2 at m/z 1781.6, (b) Hex6HexNAc5 at m/z 2228.8, (c) Hex6HexNAc5dHex1 at m/z 2374.8, and (d) Hex6HexNAc5dHex2 at m/z 2520.9. N-Glycans were ionized as phosphate adducts after on-target 3-AQ labeling. Red letters indicate product ions originating from a major isomer; blue letters indicate those from a minor isomer. Insets depict structures deduced from the negative-ion CID spectra. Reproduced with permission from ref. 35. Copyright (2012) American Chemical Society.

Fig. 4. Representative negative-ion CID spectra of desialylated N-glycans derived from the HER2 protein (SK-BR-2 cell line). CID spectra of (a) Hex7HexNAc2 at m/z 1781.6, (b) Hex6HexNAc5 at m/z 2228.8, (c) Hex6HexNAc5dHex1 at m/z 2374.8, and (d) Hex6HexNAc5dHex2 at m/z 2520.9. N-Glycans were ionized as phosphate adducts after on-target 3-AQ labeling. Red letters indicate product ions originating from a major isomer; blue letters indicate those from a minor isomer. Insets depict structures deduced from the negative-ion CID spectra. Reproduced with permission from ref. 35. Copyright (2012) American Chemical Society.