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. 2017 Apr 3;36(31):4405–4414. doi: 10.1038/onc.2017.69

Figure 2.

Figure 2

Ectopic SPZ1 expression promotes proliferation, transformation and tumor growth. (a) Effect of forced expression (SPZ1-GFP) and knockdown of SPZ1 (SPZ1 shRNAi1) on cell growth was measured at 12 h intervals. Expression of SPZ1 and exogenous SPZ1-GFP in each transformant were measured by western blotting (inside Figures). (b) Effect of forced expression (SPZ1-GFP) and knockdown of SPZ1 (SPZ1 shRNAi1) on cell proliferation was measured by BrdU incorporation into SK-Hep1 and Huh 7 cells. (c) Effect of forced and knockdown of SPZ1 on colony formation was measured in SK-Hep1 and Huh 7 cells. (d) Effect of forced and knockdown of SPZ1 on tumor growth of transformed SK-Hep1 or Huh 7 in nude mice (n=6). Internal Figures; the cancer of xenografts of pEGFP and SPZ1 shRNAi1. (e) Tumor was detected in each organ of 6- to 8-month-old SPZ1 transgenic mice prepared in the Material and methods section. Eighteen out of 60 transgenic mice generated the cancer features (n=60). Number in parentheses indicate the number of the mice with cancer characteristics. a and b, P<0.001.