Figure 4.
Subcellular localization of Ii049 in the rice cell. (A) A rice protoplast expressing Ii049-GFP showing green fluorescent signals in the nucleus. (B) The same protoplast cell of (A) showing the chlorophyll autofluorescence signal in the plastids. (C) The merged signal of (A,B). (D) The same protoplast of (C) under bright-field. Bars = 7.5 μm. (E) A rice protoplast expressing GFP showing green fluorescent signals. (F) The same protoplast cell of (E) showing the chlorophyll autofluorescence signal in the plastids. (G) The merged signal of (E,F). (H) The same protoplast of (G) under bright-field. Bars = 10 μm. This experiment was repeated three times with similar results.