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. 2017 Aug 4;8:1361. doi: 10.3389/fpls.2017.01361

Figure 5.

Figure 5

Characterization of transgenic hairy roots silencing Ii049. (A) Time course of biomass accumulation of hairy root lines WT, CK1, Ri049-1, Ri049-2, and Ri049-8. (B) Representative PCR analyses for the rolb and hpt genes and the specific gene in transgenic hairy root lines. M, DL-2000 Marker; P, the corresponding engineered bacteria (positive control); and WT, the wild-type hairy root (negative control). (C) Ii049 transcript levels. Bars indicate standard deviation. The level of significance obtained using the Student t-test is marked by the following: *P < 0.05, **P < 0.01. (D) The phenotype of Ri049 lines before (upper) and after (lower) phloroglucinol-HCl staining. (E) HPLC-MS/MS analysis of pinoresinol, lariciresinol and secoisolariciresinol of different Ri049 lines. (F) SA content in Ri049 lines. (G) Relative expression of lignan/lignin biosynthetic genes in hairy root lines WT, Ri049-1, Ri049-2, and Ri049-8. Each data point is the average of three biological replicates. Bars indicate standard deviation. The level of significance obtained using the Student t-test is marked by the following: *P < 0.05, **P < 0.01.