Figure 6.

Effects of cocaine (1 µM), the selective dopamine (DA) reuptake blocker GBR 12783 (20 nM), amphetamine (1 µM), JWH-018 (1 µM, 100 nM), and AKB48 (1 µM, 100 nM) on [³H]-DA uptake in striatal synaptosomes from CD-1 mice. The drugs were added to synaptosomes 5 min before [³H]-DA and uptake was measured for 10 min at 37°C. A same volume of drug vehicle (Kreb’s solution or ethanol) was added 5 min before [³H]-DA incubation in the control/vehicle groups, respectively. The effect of the treatments on [³H]-DA uptake is expressed as percent of control values, i.e., tritium content measured in untreated synaptosomal aliquots, always assayed in parallel (100 ± 3%, n = 4; indicated by a dashed line). Unspecific uptake was measured at 0°C. Each treatment bar represents the mean ± SEM of four determinations ran in duplicate. **p < 0.01, *p < 0.05 significantly different from GBR 12783 20 nM; °°p < 0.01, °p < 0.05 significantly different from amphetamine 1 µM; ■■p < 0.01, ■p < 0.05 significantly different from cocaine 100 nM according to one-way ANOVA followed by Newman–Keuls test for multiple comparisons.