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. 2017 Aug 4;8:188. doi: 10.3389/fendo.2017.00188

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Copyright © 2017 Lecka-Czernik, Stechschulte, Czernik, Sherman, Huang and Krings.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of ovariectomy (OVX) on body composition, bone mass, and profile of gene markers expression. Mice (4 months old, n = 8 per group) underwent either Sham surgery or OVX and were sacrificed 4 weeks after surgery. (A) Body weight change, body composition measured with NMR, and weights of gonadal WAT (gWAT) and interscapular BAT were measured at the end of experiment. (B) Analysis of trabecular bone in proximal tibia. BV/TV, trabecular bone mass; TbN, trabeculae number; TbTh, trabeculae thickness. (C) Cortical bone mass measured in midshaft tibia. Tar, total area including bone area and marrow cavity area; MrAr, marrow cavity area; CtTh, cortical thickness. (D) Marrow fat volume in proximal and distal tibia, and microcomputed tomography images of marrow adipose tissue in tibia bone stained with osmium tetroxide. (E) Profile of gene expression markers in proximal tibia. (F) Profile of gene expression markers in gWAT isolated from the same animals as in (D). In both panels, expression of Ucp1, Prdm16, Tbx1, and Dio2 was normalized to Fabp4 expression in the same sample (n = 4 tibia bones or four gWAT from four different animals per group). *p < 0.05; **p < 0.01; ***p < 0.001.

Effect of ovariectomy (OVX) on body composition, bone mass, and profile of gene markers expression. Mice (4 months old, n = 8 per group) underwent either Sham surgery or OVX and were sacrificed 4 weeks after surgery. (A) Body weight change, body composition measured with NMR, and weights of gonadal WAT (gWAT) and interscapular BAT were measured at the end of experiment. (B) Analysis of trabecular bone in proximal tibia. BV/TV, trabecular bone mass; TbN, trabeculae number; TbTh, trabeculae thickness. (C) Cortical bone mass measured in midshaft tibia. Tar, total area including bone area and marrow cavity area; MrAr, marrow cavity area; CtTh, cortical thickness. (D) Marrow fat volume in proximal and distal tibia, and microcomputed tomography images of marrow adipose tissue in tibia bone stained with osmium tetroxide. (E) Profile of gene expression markers in proximal tibia. (F) Profile of gene expression markers in gWAT isolated from the same animals as in (D). In both panels, expression of Ucp1, Prdm16, Tbx1, and Dio2 was normalized to Fabp4 expression in the same sample (n = 4 tibia bones or four gWAT from four different animals per group). *p < 0.05; **p < 0.01; ***p < 0.001.