Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) Expression strategy to produce an oligomannose-type glycoform of gp120. Kifunensine inhibits endoplasmic reticulum (ER) and Golgi mannosidase I during recombinant HIV gp120 expression resulting in predominantly Man9GlcNAc2 (Man9) N-glycans. (B) ESI-MS of Nlinked glycans released by protein N-glycanase F. (C) Tandem mass spectrometry of negative N-glycan ions (diagnostic ions for each isomer are in orange or purple). Green circles, mannose; Blue squares, GlcNAc.
