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Intact mass spectra of N332A gp120 (black) and wild-type (WT) gp120 (blue) expressed in the presence of kifunensine. The number of N-glycosylation sites at each cluster of peaks is indicated (n). WT and the N332A mutant of BG505 gp120 have a maximum N-glycosylation site occupancy potential of n = 24 and 23, respectively.
