Table 1. Antibodies used for flow cytometry analysis.
| Antibody | Clonality | Fluorophore | Dilution | Host
Animal |
Manufacturer | Catalog # | RRID |
|---|---|---|---|---|---|---|---|
| CD3 | 17A2 | FITC | 1/200 | Rat | Biolegend | 100203 | AB_312660 |
| CD8α | 53-6.7 | FITC | 1/200 | Rat | Biolegend | 100705 | AB_312744 |
| CD19 | 6D5 | FITC | 1/200 | Rat | Biolegend | 115505 | AB_313640 |
| Gr1 | RB6-8C5 | FITC | 1/200 | Rat | Biolegend | 108405 | AB_313370 |
| NK1.1 | PK136 | APC-eFluor 780 | 1/200 | Mouse | eBioscience | 47-5941 | AB_2637449 |
| CD45 | 30-F11 | Brilliant Violet 510 | 1/200 | Rat | Biolegend | 103137 | AB_2561392 |
| CD49a | Ha31/8 | Alexa Fluor 647 | 1/100 | Hamster | BD Biosciences | 562113 | AB_11153312 |
| CD49b | DX5 | PerCP-eFluor 710 | 1/200 | Rat | eBioscience | 46-5971 | AB_11149865 |
| CD127 | A7R34 | PE | 1/100 | Rat | eBioscience | 17-1271 | AB_469435 |
| NKp46 | 29A1.4 | PerCP-eFluor 710 | 1/50 | Rat | eBioscience | 46-3351 | AB_1834441 |
| Eomes | Dan11mag | PE-eFluor 610 | 1/100 | Rat | eBioscience | 61-4875 | AB_2574614 |
| Eomes | Dan11mag | PE-Cyanine7 | 1/100 | Rat | eBioscience | 25-4875 | AB_2573453 |
| Tbet | eBio4B10 | eFluor 660 | 1/100 | Mouse | eBioscience | 50-5825 | AB_10596655 |
| Tbet | eBio4B10 | PE-Cyanine7 | 1/100 | Mouse | eBioscience | 25-5825 | AB_11041809 |
| RORγt | Q31-378 | PE-CF594 | 1/100 | Mouse | BD Biosciences | 562684 | AB_2651150 |