Figure 4.

RIN1 activates EGFR signaling. (a) GSEA plot showing RIN1 expression in association with EGF signaling‐related genes in the TCGA clear cell renal cell carcinoma (ccRCC) dataset. (b) The effects of RIN1 knockdown or overexpression on phosphorylation of EGFR, AKT and ERK were evaluated by western blot analysis. (c) Correlation of expression of proteins in the TCGA RPPA data with RIN1 (x‐axis) and p‐AKT (PT308) (y‐axis). (d) Correlation of expression of proteins in the TCGA RPPA data with RIN1 (x‐axis) and p‐ERK Kinase 1 (PS217_S221) (y‐axis). “Altered” means the patients with RIN1 mRNA upregulation (n = 28) and the patients with RIN1 missense mutation (n = 1), other patients are mentioned as “Unaltered.”