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. 2017 Jul 3;108(8):1620–1627. doi: 10.1111/cas.13297

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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RIN1 interacts with Rab25 in clear cell renal cell carcinoma (ccRCC) cells. (a) 786‐O cells transfected with the Flag‐tagged Rab25 or with Flag‐tagged RIN1 construct was subjected to IP with either control IgG or Flag antibody, and the interaction of endogenous RIN1 and Rab25 was determined by western blotting with their specific antibodies, respectively. (b) Left panel: Significant positive correlations were evaluated between the levels of RIN1 and Rab25 in 20 ccRCC samples by immunohistochemistry. Right panel: IHC images representing low and high expression of RIN1 and Rab25. (c) Kaplan–Meier analysis of the mRNA expression levels of RIN1 correlated with the overall survival (OS) of 533 patients with ccRCC in the TCGA cohort. (d) Kaplan–Meier analysis of the mRNA expression levels of Rab25 correlated with the OS of 533 patients with ccRCC in the TCGA cohort.