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. 2017 Feb 28;21(8):1619–1635. doi: 10.1111/jcmm.13091

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© 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Rapamycin suppresses cell‐cycle‐associated cyclins in vitro. (A) Cell line E8 and its material derived cell line D3 were incubated with 0, 1 or 10 nM rapamycin for 12 hrs. Western blots of the lysates of D3 and E8 cells showed that rapamycin decreased the expression of cyclins A, B, D1 and E in a dose‐dependent manner. (B) D3 and E8 cells were incubated with 10 nM rapamycin for 0, 6 or 12 hrs. Western blots of the cell lysates showed that rapamycin significantly decreased the expression of cyclins A, B, D1 and E in a manner dependent on the duration of incubation. (C–F) Normalized quantitative analysis using the densitometry values from the Western blots in (A). (G–J) Normalized quantitative analysis using the densitometry values from the Western blots presented in (B). N.S. = No significance; *P < 0.05; **P < 0.01.