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. 2017 Jul 29;108(8):1701–1714. doi: 10.1111/cas.13295

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Profiling of V‐set and immunoglobulin domain containing 1 (VSIG1) protein expression in cell lines, and generation of VSIG1‐overepresssing cell lines. (a) Panel of VSIG1 expression detected by Western blot in human cell lines of gastric cancer, non‐small‐cell lung cancer, and esophageal cancer. (b) Expression levels of VSIG1 mRNA of two variants in MKN45 cells, which endogenously expressed VSIG1, were compared by quantitative RT‐PCR. (c) Establishment of cells stably overexpressing VSIG1 variant 2 in gastric cancer cell lines MKN1 and MKN28, lung cancer cell line H1299, and esophageal cancer cell line KYSE150. (d) Subcellular localization of VSIG1 was visualized by immunofluorescence using the anti‐VSIG1 antibody. Membranous localization of VSIG1 (red) was observed.