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. 2017 Jul 18;108(8):1584–1593. doi: 10.1111/cas.13289

Figure 1.

Figure 1

Chemokine (CC motif) ligand 18 (CCL18) promoted epithelial–mesenchymal transition in oral squamous cell carcinoma. (a,b) HSC‐6 and CAL33 cells were cultured in the absence or presence of CCL18 (20 ng/mL) for 48 h, then their migration and invasion abilities were examined by Transwell assay. Representative pictures and the mean number of cells that migrated (a) or invaded (b) to the lower surface are shown. (c) Epithelial–mesenchymal transition ‐associated markers, E‐cadherin and N‐cadherin, were examined by Western blotting after cells were treated with or without CCL18 (20 ng/mL) for 2 or 3 days. α‐Tubulin was used as an internal control. (d) Immunofluorescence staining of E‐cadherin (red) and N‐cadherin (green) was indicated in HSC6 and CAL33 cells with or without CCL18 for 3 days. Nuclear DNA was stained with DAPI (blue). Data are presented as the means ± SEM of at least three independent experiments, *P < 0.05 compared to the non‐target control (NC).