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. 2017 Jun 9;21(8):1572–1583. doi: 10.1111/jcmm.13087

Figure 5.

Figure 5

CM from laminin‐cultured PDMCs possesses an enhanced capacity to suppress mCardio apoptosis. (A) mCardios were treated with treated with TNF‐α alone or mixed with CM from PDMCs cultured on uncoated plates (PDMC‐PBS‐CM), plates coated with various proteins as indicated including laminin (PDMC‐lam‐CM) or mouse cardiomyocyte CM (mCardio‐CM). After 48 hrs, cardiomyocytes were stained for annexin V and propidium iodine (PI) and analysed by flow cytometry. Representative graphs and (B) quantitative results are shown. (C) Activated/cleaved caspase‐3 in TNF‐α‐treated mCardios after the addition of PDMC‐CM from uncoated plates (PBS) or plates coated with various proteins as indicated was assayed by Western blotting. Data are shown as the mean ± S.E.M. of three independent experiments. *P < 0.05; **P < 0.01; and ***P < 0.005 compared to TNF‐α only; #P < 0.05 compared to PDMC‐PBS‐CM.