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. 2017 Jun 19;108(8):1565–1573. doi: 10.1111/cas.13280

Figure 3.

Figure 3

Overexpression of dual‐specificity tyrosine‐regulated kinase 2 (DYRK2) inhibits cell activity in HCT116 colorectal cancer cell line. (a) HCT116‐E2 cells were transfected with WT DYRK2 (Flag‐DYRK2‐WT [WT]), kinase‐dead DYRK2 mutant (K178R) (Flag‐DYRK2‐KR [KR]), or Flag vector as control (Flag). Expression levels of DYRK2, E‐cadherin, Snail, and c‐Myc were analyzed by Western blotting. (b) Flag, WT, or KR cells were immunostained with anti‐Snail or anti‐E‐cadherin antibodies. (c,d) Association between DYRK2 expression and cell proliferation motility of colorectal cancer. Colony formation assay (c) and cell growth analysis by MTS (d) were carried out. Data are shown as relative values based on the first day and are expressed as the mean ± SD (n = 5). *P < 0.05. (e,f) Stable overexpression of DYRK2 leads to inhibition of invasion and migration. Migration (e) and invasion (f) potentials were analyzed by a migration assay and an invasion assay, respectively. Numbers of migrating and invading cells were counted. Data are displayed as mean ± SD (n = 3). *P < 0.05.