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Investigation of the aggregation behavior of conjugates 2 and 4, Lewis X conjugate, and BSA, in the presence of either 10 mM CaCl2 (A) or 10 mM MgCl2 (B). On addition of divalent cation (1 M, 5 μl) to a solution (10 μM, 495 μl) of test molecule, the tube was mixed and the absorbance was zeroed. The absorbance was then measured at 340 nm for 6,000 s.
