Table 1.

Effect of bile salts, steroids, and anionic drugs on Ostα/Ostβ-mediated [³H]taurocholate and [³H]estrone sulfate uptake

	[3H]Taurocholate uptake (% control)	[3H]Estrone sulfate uptake (% control)
Bile salts
Control	100  ± 4	100  ± 4
Lithocholic acid, 200 μM	95  ± 6	94  ± 4
Lithocholic acid sulfate, 200 μM	40  ± 1*	27  ± 1*
Glycolithocholic acid, 200 μM	124  ± 8	107  ± 3
Glycolithocholic acid sulfate, 200 μM	72  ± 4*	49  ± 2*
Taurolithocholic acid, 200 μM	30  ± 2*	36  ± 5*
Taurolithocholic acid sulfate, 200 μM	24  ± 3*	22  ± 4*
Scymnol sulfate, 200 μM	54  ± 5*	70  ± 5*
Glycodeoxycholic acid, 200 μM	82  ± 6	110  ± 4
Taurodeoxycholic acid, 200 μM	39  ± 5*	103  ± 5
Taurocholate, 500 μM	70  ± 6*	95  ± 6
Steroids
Control	100  ± 4	100  ± 4
Estrone sulfate, 200 μM	60  ± 1*	62  ± 5*
Spironolactone, 200 μM	24  ± 1*	36  ± 2*
Digoxin, 500 μM	74  ± 5*	56  ± 7*
Ouabain, 1 mM	96  ± 8	100  ± 8
17β-Estradiol, 200 μM	80  ± 3*	100  ± 4
Testosterone, 200 μM	78  ± 7*	84  ± 3*
Dexamethasone, 200 μM	103  ± 6	97  ± 8
Other anions
Control	100  ± 4	100  ± 4
Sulfobromophthalein, 100 μM	44  ± 5*	69  ± 2*
Bilirubin ditaurate, 500 μM	43  ± 2*	83  ± 9
Probenecid, 1 mM	53  ± 8*	61  ± 8*
p-Aminohippurate, 500 μM	71  ± 7*	111  ± 3
S-dinitrophenyl glutathione, 500 μM	80  ± 7	99  ± 6
Indomethacin, 200 μM	47  ± 6*	50  ± 3*

Uptake of 20 μM [³H]taurocholate and 50 nM [³H]estrone sulfate was measured for 30 min and 1 hr, respectively, in the absence (control) and presence of the indicated compounds. Data are expressed as a percent of the control values ± SE (n = 3–4). 

^*, Significantly different from control, P < 0.05.