Table 2.

BRAF mutations in melanoma tumours

Primary melanomas N mut/total N (%)	Metastases N mut/total N (%)	Materials	Sub‐dissection	Detection method	References
6/9 (67)		P	No	D,G,I	Davies et al., 2002
22/35 (63)a		F	No	D,G,I	Brose et al., 2002
7/28 (25)	8/13 (62)	P	Yes	D,I	Dong et al., 2003
	33/77 (43)	F	No	D,I	Gorden et al., 2003
4/5 (80)	37/55 (67)	P	Yes	D,I	Pollock et al., 2003
7/25 (28)	26/60 (38)	F	No	D,H	Alsina et al., 2003
14/25(52)		P	Yes	D,H,I	Uribe et al., 2003
2/10 (20)	14/34 (41)	P	No	D,G,I	Cruz et al., 2003
41/71 (58)	43/88 (49)	P	Yes	D,F,I	Omholt et al., 2003
32/115		P	No	D,I	Maldonado et al., 2003
12/35 (34)		P	No	D,F,I	Kumar et al., 2003a
	26/38 (68)	P	No	DF,I	Kumar et al., 2003b
19/50 (38)		P	Yes	D,F,I	Deichmann et al., 2004
8/15 (53)	6/22 (27)	F	No	D,F,I	Reifenberger et al., 2004
39/114 (34)	36/86 (42)	P	Yes	D,I	Houben et al., 2004
18/59 (31)	39/68 (57)	P	Yes	D,I	Shinozaki et al., 2004
25/36 (69)	43/79 (54)	F	No	D,H	Goydos et al., 2005
17/37 (46)		P	Yes	D,I	Thomas et al., 2004
9/12 (75)		P	No	D,F,I	Saldanha et al., 2004
15/51 (29)b	8/18 (44)	P	Yes	D,F,I	Akslen et al., 2005
13/52 (25)		P	No	D,G,I	Lang and MacKie, 2005
3/27 (11)c		P	No	D,I	Curtin et al., 2005
22/37 (60)d
33/58 (57)		P	Yes	D,I	Goel et al., 2006
	156/294 (53)e	F	No	D,K	Edlundh‐Rose et al., 2006
8/15 (53)		P	Yes	D,I	Lassacher et al., 2006
17/62 (27)		P	Yes	D,I	Poynter et al., 2006
	53/97 (55)	F	No	D,G,I	Ugurel et al., 2007
Total 393/983 (40)	528/1029 (51)

Materials: F=fresh frozen tissues; P=formalin fixed, paraffin embedded tissues; manual or laser capture sub‐dissection: yes/no=Detection Method: D PCR or RT PCR; E allele specific oligonucleotide hybridization; F SSCP or DGGE; G HPLC/capillary techniques; H RFLP dependent or allele specific amplification; I nucleotide sequencing; K pyrosequencing.

^aEarly passage melanoma cell lines.

^bNM only.

^cContinuously sun exposed sites, mainly LMM.

^dIntermittently sun exposed sites, mainly SSM.

^eIncluding 39 primary melanomas.