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. 2007 Mar 14;1(1):55–71. doi: 10.1016/j.molonc.2007.02.002

Figure 4.

Figure 4

Meox2 is required for optimal p21 levels after TGF‐β stimulation. (A) Quantitative real time RT‐PCR analysis of MEOX2 and GAPDH mRNA expression in HaCaT cells transfected with the indicated siRNAs and stimulated with 5ng/ml TGF‐β1 for 0 or 16h. (B) Quantitative real time RT‐PCR analysis of p21 and GAPDH mRNA expression in the same HaCaT cells of panel A. (C) Immunoblot analysis of endogenous p21 and β‐tubulin protein expression in HaCaT cells transfected and stimulated under identical conditions as in panel A. (D) Semi‐quantitative RT‐PCR analysis of endogenous MEOX2, ID2, PAI‐1 and GAPDH levels in HaCaT cells transfected and stimulated under identical conditions as in panel A.