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. 2007 Mar 14;1(1):55–71. doi: 10.1016/j.molonc.2007.02.002

Figure 7.

Figure 7

Meox2 and Smad proteins interact and cooperate in inducing endogenous p21 levels. (A) Co‐immunoprecipitation assays in HaCaT cells infected with the indicated adenoviruses (MOI 200) and stimulated with 5ng/ml TGF‐β1 for 2h. Anti‐HA (Meox2) immunoprecipitation (IP) was followed by immunoblot (IB) with antibodies against Flag (Smads) and HA (Meox2). Immunoblots of the corresponding total extracts with the two antibodies are shown below. (B) Immunoblot analysis of endogenous p21 expression in HaCaT cells infected with the indicated adenoviral MOI for 32h and stimulated with 5ng/ml TGF‐β1 for another 16h. Anti‐HA immunoblot shows the expression of the infected Meox2 and anti‐Flag immunoblot shows the expression of Smad3 and Smad4. (C) Immunoblot analysis of endogenous p21 protein expression in MDA‐MB‐468 cells infected with the indicated adenoviruses (MOI 100) for 48h. Anti‐HA immunoblot shows the expression of the exogenous Meox2 and β‐tubulin serves as protein loading control.