Table 3.
Protein identification by mass spectrometrya
| Protein name | Acc number (SwissProt)b | Acc number (UniGene)c | Gene Map Locus | Mm/pId | Scoree | Coverage (%) | Number of recognized peptides |
|---|---|---|---|---|---|---|---|
| Cytokeratin 7 | P08729 | KRT7/SCL | 12q12–q13 | 51/5.5 | 267 | 43 | 25 |
| Cytokeratin 8 | P05787 | KRT8/CYK8 | 12q13 | 53.5/5.5 | 238 | 40 | 40 |
| Cytokeratin 14 | P02533 | KRT14 | 17q12–q21 | 51.6/5.1 | 200 | 43 | 16 |
| Cytokeratin 15 | P19012 | KRT15 | 17q21.2 | 49/4.7 | 222 | 35 | 17 |
| Cytokeratin 18 | P05783 | KRT18/CYK18 | 12q13 | 48/5.3 | 206 | 34 | 18 |
| Cytokeratin 19 | P08727 | KRT19 | 17q21.2 | 44/5.04 | 316 | 54 | 26 |
| Fatty acid binding protein, adipocyte (A‐FABP) | P15090 | FABP4 | 8q21 | 14.7/6.8 | 130 | 45 | 8 (PSD: 935.48) |
| Glycerol‐3‐phosphate dehydrogenase [NAD+], cytoplasmic, GPDH‐C | P21695 | GPD1 | 12q12–q13 | 38/5.8 | 304 | 53 | 21 (PSD: 1556.79) |
| MRP14 (S100A9) | P06702 | S100A9/CAGB, MRP14 | 1q12–q22 | 13.5/5.7 | 148 | 60 | 8 |
| 14‐3‐3‐Sigma (Stratifin) | P31947 | SFN/HME1 | 1p36.11 | 27.8/4.8 | 90 | 35 | 8 |
a
Protein identifications from tumour biopsy, apocrine microcyst from patient 23, and MCF‐10A cells are presented.
b
Whenever possible, the SwissProt accession number was used.
c
UniGene accession number was used.
d
The Mr and pI were calculated directly from the sequences.
e
Ions score is −10*Log (P), where P is the probability that the observed match is a random event. The protein score exceeded the threshold significance score of 74 (p < 0.05) in all cases and therefore, all identifications are confident.