Table 1.

Primers used for RT‐PCR and qPCR analyses

Gene	Specificity	Forward primer 5′→3′	Reverse primer 5′→3′	Product size (bp)
TIMP‐1	All exon‐skipping variantsa	Forward exon 1 CCCTAGCGTGGACATTTATC	Reverse exon 6 AAGGTGACGGGACTGGAAG	648 (full‐length)
TIMP‐1	Full‐length+del‐2	Forward exon 1 CCCTAGCGTGGACATTTATC	Reverse exon 3 GGTATAAGGTGGTCTGGTTG	263+134
TIMP‐1	Full‐length+del‐5	Forward exon 4 ACTTCCACAGGTCCCACAAC	Reverse exon 6 AAGGTGACGGGACTGGAAG	252+127
TIMP‐1	Full‐lengthb	Forward exon 2 CTTCTGGCATCCTGTTGTTG	Reverse exon 3 GGTATAAGGTGGTCTGGTTG	153
TIMP‐1	Del‐2b	Forward exon 1/3 CCCAGAGAGACACCAGAGTCA	Reverse exon 4 GTGGGACCTGTGGAAGTATC	196
GAPDH	Full‐length	CAATGACCCCTTCATTGACC	TTCACACCCATGACGAACAT	309

^aExcept variants lacking exon 1 or 6.

^bqPCR assay.