Figure 10. Memory CCR6⁺CD4⁺ T cells infiltrating the colons of HIV+ART individuals express high levels of CCR5, integrin β 7, and phosphorylated mTOR.

Matched blood and sigmoid biopsies were collected from HIV-infected individuals with undetectable plasma viral load (<40 HIV-RNA copies/ml) under ART (Supplemental Table 5; COL 1–10). Cells were extracted and stained with a cocktail of specific Abs. (A) The gating strategy for the flow cytometry identification of CD3⁺CD4⁺CD45RA^– T cells. (B and C) The surface expression of CCR5 and (D and E) integrin β7 and (F and G) the intracellular expression of phosphorylated mTOR on CCR6⁺ and CCR6^– subsets from sigmoid biopsies versus PBMCs in one representative donor (B, D, and F) and statistical analysis in all donors (C, E, and G). Paired t test values are indicated.