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. 2017 Aug 3;2(15):e93230. doi: 10.1172/jci.insight.93230

Figure 7. mTOR inhibitors counteract the effect of ATRA on HIV permissiveness in sorted CCR6+ and CCR6– T cells.

Figure 7

Memory CCR6+ and CCR6– T cells were sorted as depicted in Figure 1 and stimulated via CD3/CD28 in presence of ATRA or ATRA and INK128 (50 nM) for 4 days. Then, cells were exposed to replication-competent T/F HIV THRO (25 ng HIV-p24/106 cells). Further, cells were cultured for 9 additional days in presence of ATRA or ATRA and INK128. (A) Absolute levels of integrated HIV DNA at day 3 after infection and (B) HIV-p24 levels in cell culture supernatants at day 6 after infection (n = 3, mean ± SD of triplicate wells). Each symbol represents one experimental triplicate value in individual donors. Ordinary 1-way ANOVA with Tukey’s multiple comparisons test (**P < 0.01; ***P < 0.001; ****P < 0.0001).