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. 2017 Aug 3;2(15):e93358. doi: 10.1172/jci.insight.93358

Figure 2. Requirement for TRPC3 in DOX-induced Nox2 upregulation.

Figure 2

(A) Representative fluorescence images (left) and semiquantitative results of fluorescence intensities (right) in mouse hearts stained with hypoxyprobe (n = 3). Scale bar: 40 μm. (B and C) Western blots (B) and quantitative results (C) for Nox2, HIF1α, HO-1, and GAPDH in C57BL/6J mouse hearts 2 weeks after DOX (n = 3). (D) Relationship between heart weight and Nox2 abundance. (E and F) Representative Western blots (E) and quantitative results (F) for Nox2, HIF1α, and GAPDH in 129Sv-background TRPC3+/+ and TRPC3–/– mouse hearts (n = 3). (G) Relationship between heart weight and Nox2 abundance. Data are shown as the mean ± SEM. Significance was determined using unpaired t test analysis in A and C, and one-way ANOVA followed by Tukey’s comparison test in F. *P < 0.05.