Skip to main content
. 2017 Aug 3;2(15):e92176. doi: 10.1172/jci.insight.92176

Figure 3. Tandem mass spectrometric analysis of proteins and quantification of ABCA1 CEC activity in mouse plasma fractionated by sequential high resolution SEC and ion-exchange chromatography.

Figure 3

Plasma (300 μl) of wild-type mice or Apoa1–/– mice was fractionated by size-exclusion chromatography (SEC) on a Superdex 200 column at a flow rate of 0.35 ml/min. The late-eluting peak of material with ABCA1 cholesterol efflux capacity (CEC) activity (fractions 15–23) from 3 different animals was pooled, concentrated, and then subjected to cation-exchange (A and E) or anion-exchange (C and G) chromatography. ABCA1 CEC was measured for each fraction. Proteins in each fraction were quantified by tandem MS analysis with spectral counting (the number of unique peptides detected for each protein). Lines represent Pearson’s correlation between ABCA1 CEC, and spectral counts in each fraction (B, D, F, and G).