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. 2017 Aug 3;2(15):e92176. doi: 10.1172/jci.insight.92176

Figure 5. ABCA1 CEC activity of plasma wild-type mice (●) and plasminogen-deficient mice (○).

Figure 5

ABCA1 cholesterol efflux capacity (CEC) activity was quantified using [3H]cholesterol-labeled BHK cells with and without induction of ABCA1 expression. (A) Plasma fractionated by high-resolution size-exclusion chromatography (SEC). Results are the mean of duplicate determinations of pooled plasma obtained by combining equal volumes of plasma from 5 different mice. (B) Area under the curve for fractions 15–25 of the data in panel A. HDL eluted in fractions 7–14. (C) Macrophage CEC and ABCA1 CEC of control and plasminogen-depleted (PLG-depleted) human plasma. Four human plasma samples were depleted [PLG(–)], not depleted [PLG(+)], or PLG depleted and supplemented with 1 μg/ml PLG. PLG was depleted by L-lysine Sepharose affinity chromatography. P < 0.05 for (A) PLG(+) versus PLG(–); (B) PLG(+) versus PLG(–) + 1 μg/ml PLG; (C) PLG(–) versus PLG(–) + 1 μg/ml PLG.