Table II. Agronomic traits of the TaTAR2.1-3A overexpression wheat plants, their azygous control plants, and wild-type plants grown under high-N (18 g m⁻²) and low-N (0 g m⁻²) conditions in the field trial.

TaOE2 and TaOE8 indicate overexpression lines, and TaOE2- and TaOE8- indicate their respective azygous control lines separated from the T2 plants. PH, Plant height (cm); Biomass, biomass per plant (g per plant); GY, grain yield (g per plant); SN, spike number per plant; GNS, grain number per spike; TGW, thousand-grain weight (g). High N represents the normal N condition for wheat growth in the field. Low N represents low-N treatment. Data are means ± se of four biological replicates. Each replicate contained at least 15 plants. Asterisks indicate statistically significant differences at P < 0.05 (*) and P < 0.01 (**).

Treatment	Genotype	Trait
		PH	Biomass	GY	SN	GNS	TGW
High N	KN199	63.55 ± 1.10	41.24 ± 2.29	19.93 ± 2.95	9.76 ± 1.85	40.25 ± 1.82	50.73 ± 1.24
	TaOE2	67.21 ± 2.57*	50.10 ± 3.55**	21.33 ± 2.55*	10.58 ± 1.41*	39.23 ± 2.41	50.25 ± 2.16
	TaOE2-	62.75 ± 0.50	41.77 ± 2.22	20.55 ± 3.44	9.35 ± 0.74	42.26 ± 2.65	52.02 ± 3.11
	TaOE8	68.50 ± 2.35*	45.94 ± 2.14*	21.52 ± 3.58*	10.15 ± 2.67*	40.44 ± 1.12	52.40 ± 2.26
	TaOE8-	62.77 ± 4.06	41.84 ± 2.25	20.26 ± 3.08	9.26 ± 1.41	42.86 ± 1.58	51.28 ± 1.63
Low N	KN199	62.25 ± 0.95	33.74 ± 2.49	17.80 ± 3.02	8.74 ± 0.87	38.11 ± 2.49	53.40 ± 1.26
	TaOE2	65.63 ± 3.07*	36.63 ± 3.28*	19.29 ± 2.32*	10.50 ± 2.34*	36.70 ± 1.35	50.70 ± 1.26
	TaOE2-	63.50 ± 2.44	34.69 ± 3.04	17.76 ± 1.74	9.15 ± 0.61	36.79 ± 1.05	52.80 ± 1.36
	TaOE8	65.20 ± 2.48*	36.39 ± 2.48*	18.69 ± 2.48*	9.64 ± 1.36*	35.92 ± 1.02	53.39 ± 0.99
	TaOE8-	61.50 ± 4.93	34.32 ± 3.73	17.45 ± 1.59	8.67 ± 0.62	36.83 ± 1.48	52.80 ± 2.52