. 2017 Jun 23;174(4):2023–2037. doi: 10.1104/pp.17.00489

Table I. Summary of mutant lines from the LRR-XII library.

Mismatches between the spacer and M82 target sequences are underlined. Alleles are combined data from T0 and T1 plants. The genotype of the plants is indicated. Active indicates that Cas9 is actively producing new alleles in those lines. NA, Not applicable; WT, wild type.

Line	Target + PAM	Target	LRR-XII Family?	T0 and T1 Alleles	Genotype	Notes
LRR-1	`GAAGATATGGTTACTCATGTTGG`	Solyc07g016010	Yes	−4 bp deletion/WT, active	M82sp+	T1: some weak plants, low seed set, sterility
LRR-2	`GTCACCACAAAGTGCATCATTGG`	Solyc06g006040	Yes	−4/+609 (from Solyc06g006020)	M82sp+	T0 and T1: high seed weight, low fruit set
	`GTCACCACAAAGTGCATCATCGG^a`	Solyc06g006020	Yes	−9/larger deletion
	`GTCACCACAAAGTGCATCATCGG^a`	Solyc06g006050	Yes	−1/no amplification (276 upstream)
	`GTCACCACAAAGTGCATCATCGG^a`	Solyc06g006070	Yes	−9/−8 (315 upstream)
LRR-3	`No gRNA`	N/A	N/A	N/A	M82sp+
LRR-4	`GGCAATCTCAGCTTCCTAACAGG`	Solyc10g085110	Yes	WT	M82sp+	T1: leaf development (1/4)
	`GAGATGCAACATCCACATCTGGG`	Solyc03g118330	Yes	−1/−2,112 bp
	`GCAGGTGACCAAGTCCATAAGGG`	Solyc04g014900	Yes	−1/no amplification (unresolved)
LRR-5	`GAAAGATCAAAAATTTCAGCAGG`	Solyc07g018180	Yes	WT, sequence different from the reference	M82sp+
LRR-6	`GGATTAGTGATTCCTTTCCGGGG`	Solyc06g006020	Yes	−2/−3/−7	M82sp+	Mostly parthenocarpic fruit with small aborted seed; one T1 plant
LRR-6	`GGATTAGTGATTCCTTTCCGAGG^a`	SL2.50ch00:17387212	No	−5/−6/WT	M82sp+
LRR-7	`GAACTCTCATCATTGTCGGAGGG`	Solyc03g096190	Yes	WT	M82sp+
LRR-8	`GCTTTGGAATATCTTCACCACGG`	Solyc03g006100	Yes	−1/−5	M82	T1: serrated cotyledons
LRR-9	`GTAGGGATAGTGTCAACTATGGG`	Solyc08g075620	Yes	−7/−1/−3	M82sp+	Sterile
LRR-10	`GCTTTAGACTATCTCCATAAAGG`	Solyc02g068880	Yes	−2/WT/−∼330	M82sp+	Sterile
LRR-11	`GTGTACAAAGGTGCATTGTTTGG`	Solyc04g014890	Yes	+1 homozygous	M82	T1: stunted, bleached plants (1/4)
	`GTGTACAAAGGTGCATTGTTTGG^a`	Solyc04g014650	Yes	−2/+1
	`GTGTACAAAGGCACATTGTTTGG^b`	Solyc04g014900	Yes	+1/WT
	`GTGTACAAAGGCACATTGTTTGG^b`	Solyc04g015000	Yes	WT
	`GTGTACAAAGGCACATTGTTTGG^b`	SL2.50ch04 6829670	No	Active
LRR-12	`GAAAGCAACTTGCTTGGTAACGG`	Solyc02g068830	Yes	WT	M82sp+
LRR-13	`GCAGGCAAAAAACCGTCCAAGGG`	Solyc02g068820	Yes	WT	M82sp+
LRR-14	`GTAGGGATAGTGTCAACTATGGG`	Solyc08g075620	Yes	WT	M82	T1: no anthocyanin (1/4)
LRR-15	`GGCTACAGCTTGTTAATAGTCGG`	Solyc06g005880	Yes	−7/−87	M82sp+
LRR-16	`GGCCTATTCCGAGTTCATTCGGG`	Solyc02g031790	Yes	−4/−5	M82sp+
LRR-17	`GGTCAGATACCTAGTACAATCGG`	Solyc08g075600	Yes	−1/−8	M82
LRR-18	`GTCATTTTCATCCTTTCGACCGG`	Solyc02g072400	Yes	WT	M82
LRR-19	`GGCGGCCAACCATCCACACTTGG`	Solyc10g085120	Yes	−1/−3/−4/−5	M82sp+	Likely tetraploid
LRR-20	`GAGATTTGAATTTGAGAAGCTGG`	Solyc02g072380	Yes	WT	M82sp+
LRR-21	`GAGATATTAAACAGTGACGCGGG`	Solyc04g015980	Yes	+1/−1/−4	M82sp+	Mostly parthenocarpic fruit with small aborted seed; one T1 plant
LRR-22	`GCAGCAGCTTGTTATGACTTTGG`	Solyc06g006030	Yes	WT	M82sp+
LRR-23	`GGTGAAATTCCTGCCGAGCTGGG`	Solyc02g070890	Yes	−2 homozygous	M82sp+	T1: all are insensitive to flg22 (expected)
LRR-23	`GGTGAAATTCCTGCCGAACTAGG^b`	Solyc02g070910	Yes	Somatic active	M82sp+	T1: all are insensitive to flg22 (expected)
LRR-24	`GCAGGCAAAAAACCGTCCAAGGG`	Solyc02g068820	Yes	WT	M82sp+	T1: some plants light bleaching, slow-growing plants, low fruit set
LRR-24	`GTGAGGTTCCCAATAGAAGGCGG`	Solyc07g018190	Yes	WT	M82sp+
LRR-25	`GTGCAGTTAGATAAACCAGGGGG`	Solyc02g072440	Yes	−3/−4/−9/−10	M82sp+	T0 would not self, many parthenocarpic fruit, few seeds from LRR-25 × M82sp+

Unintended targets that were missed during gRNA design.

Off-targets to the gRNA sequence.