Figure 3.

Subcellular localization of native and N terminus truncated oleosins of P. patens and Arabidopsis in tobacco cells after stable transformation. A, In linear portions (following those described in Fig. 2 legend), the native and N terminus-truncated oleosins of P. patens (OLE, uptop) and Arabidopsis (AtT, bottom). ΔN23, ΔN28, ΔN31, ΔN6, and ΔN10 indicate the number of residues deleted from the N terminus (see “Results” for explanation). B, Images of a portion of a cell after expression of DNA constructs encoding various oleosins with the C terminus attached to GFP. GFP fluorescence and ER-Tracker-Red staining of ER were monitored with CLSM. Bars = 10 μm.