Figure 4.

AtZAR1^CC-YFP induces constitutive HR when inducibly expressed in N. benthamiana. A. tumefaciens carrying constructs expressing Empty Vector (EV) or AtZAR1 domains with a C-terminal YFP fusion was syringe infiltrated into N. benthamiana leaves. The experiments were performed at least three times with similar results. A, For each construct, the percentage of leaves with a strong HR (black bars), medium HR (hatched bars), weak HR (dotted bars), or no HR (white bars) is shown. B and C, Electrolyte leakage was measured 15 (T0) and 36 (T21) hours after dexamethasone induction. The error bars indicate the se from six repetitions. The letters beside the bars indicate significance groups, as determined by a one-way ANOVA comparison followed by a Tukey’s post hoc test (P value ≤ 0.05). C, Electrolyte leakage for AtZAR1^CC-YFP or EV-YFP was grouped into four phenotypic categories based on the macroscopic HR (none, weak, medium, and strong) observed 36 h after dexamethasone induction.