Figure 4.

Expression of the known key trichome formation-regulating transcription factor genes in the wild type and gain- and loss-of- function mutants of NTL8. A, Expression of the known key trichome formation regulating transcription factor genes in the Ws wild-type, the gpa1-2 mutant, and the ntl8-1D/gpa1-2 mutant seedlings. Total RNA was isolated from 10-d-old seedlings grown on 0.5× MS plates and qRT-PCR was used to examine the expression of the key transcription factor genes involved in the regulation of trichome formation. ACT2 was used as reference gene, and expression of each gene in the Ws wild-type seedlings was set as 1. Data represent the mean ± sd of three replicates. B, Expression of TRY and TCL1 in the Col wild type, the 35S:HA-NTL8 transgenic plant, and the ntl8 loss-of-function mutants. Total RNA was isolated from 10-d-old seedlings grown on 0.5× MS plates, and qRT-PCR was used to examine the expression of TRY and TCL1. ACT2 was used as reference gene, and expression of each gene in the Col wild-type seedlings was set as 1. Data represent the mean ± sd of three replicates. C, Expression of NTL8, TRY, and TCL1 in the internodes of the main inflorescence stem of Col wild-type plants. Total RNA was isolated from different internodes of soil-grown Col wild-type plants, and qRT-PCR was used to examine the expression of NTL8, TRY, and TCL1. ACT2 was used as reference gene, and expression of each gene in first internode was set as 1. Data represent the mean ± sd of three replicates.