Figure 7.

HA-PEX6 expression rescues IBA resistance in lateral root production, PTS2-processing defects, and low PEX1 levels in pex1-2. A, Seedlings were grown in the absence of hormone for 4 d and then transferred to either medium without hormone or supplemented with IBA for an additional 4 d. The numbers of lateral roots and root lengths were measured, and the ratio is shown. Error bars show se (n = 20). Significant differences (one-way ANOVA, P < 0.001) for each growth condition are marked with different letters above the bars. Data are representative of three replicates. B, Micrographs of representative 8-d-old IBA-treated seedlings from A. Bar = 2 mm. C, Twenty-one-day-old plants were prepared for immunoblot analysis and serially probed with antibodies recognizing the indicated proteins. PMDH is translated as a precursor (p) with a PTS2 that is cleaved inside the peroxisome to yield mature (m) protein. HSC70 is a loading control. The asterisk indicates a cross-reacting band. Wt, Wild type.