Figure 3.

Effects of exogenously supplied OsHAD1 with phytate on the growth of Pi-starved wild-type rice plants. A, Improvement in growth of 15-d-old Pi-deficient wild-type rice seedlings after 7 d of recovery with phytate (100 µm) or phytate + OsHAD1 (125 ng of OsHAD1 per 1 mL of Yoshida medium supplemented with 100 µm phytate). Each stack consists of five seedlings. Bar = 10 cm. B to I, Leaf number (B), tiller number (C), fresh root biomass (mg plant⁻¹; D), fresh shoot biomass (mg plant⁻¹; E), root dry biomass (F), shoot dry biomass (G), and total P content (µg plant shoot⁻¹; H) in dried shoot as well as total P content (µg plant root⁻¹; I) in dried roots of wild-type rice seedlings grown under Pi sufficient (+P), Pi deficient (−P), phytate recovered (Phytate), and phytate plus OsHAD1 (Phytate+OsHAD1) recovered conditions. –P and +P plants were provided 1 µm Pi (NaH₂PO₄) and 320 µm Pi, respectively, for 22 d in Yoshida medium. Error bars indicate se (n = 20). Student’s t test was used to calculate significance. Asterisks indicate P ≤ 0.05 (*), P ≤ 0.01 (**), and P ≤ 0.001 (***).