Table II. Steady-state kinetic parameters of the recombinant DCA synthase.
Data are means ± sd of triplicate measurements.
| Km | kcat | kcat/Km | ||
|---|---|---|---|---|
| Substrate Structures | Product Structures | μm | s−1 | s−1 μm−1 |
 |
 |
1.19 ± 0.20 | 6.53 ± 0.12 | 5.61 ± 0.82 |
| Grifolic acid | DCA | |||
 |
Product not detected | - | - | - |
| Grifolin | ||||
 |
Product not detected | - | - | - |
| Cannabigerolic acid | ||||
 |
 |
66.1 ± 12.7 | 2.14 ± 0.39 | 0.032 ± 0.001 |
| Cannabigerorcinic acid | Cannabichromeorcinic acida | |||
 |
 |
7.46 ± 1.85 | 0.131 ± 0.029 | 0.018 ± 0.002 |
| 3-Geranylgeranyl orsellinic acid | Diterpenodaurichromenic acida |
a
The absolute configuration of these products was not determined.