Table IV. Distribution of DCA synthase activity between culture supernatant and cell extract prepared from the transgenic tobacco BY-2 cell culture expressing a DCA synthase-GFP fusion protein.

Data are means ± sd of triplicate measurements.

Enzyme Preparation	Total Activity	Total Protein	Specific Activity
	pkat in mL of culture	mg in mL of culture	pkat mg−1 protein
Culture supernatant	161.6 ± 7.5	0.096 ± 0.013	1,678 ± 35.8
Cell extract	0.556 ± 0.051	0.643 ± 0.034	0.862 ± 0.041