Figure 2.

TaVIT2 facilitates iron and manganese transport. A to C, Yeast complementation assays of TaVIT1 and TaVIT2 in Δccc1 (A), Δpmr1 (B), and Δzrc1 (C) compared with yeast that is wild type (WT) for these three genes. The yeast (Sc) CCC1, PMR1, and ZRC1 genes were used as positive controls. Cells were spotted in a 4-fold dilution series and grown for 2 to 3 d on plates with or without 7.5 mm FeSO₄ (Δccc1), 2 mm MnCl₂ (Δpmr1), or 5 mm ZnSO₄ (Δzrc1). D, Immunoblots of total and vacuolar protein fractions from yeast cells expressing hemagglutinin (HA)-tagged TaVIT1 or TaVIT2. The HA tag did not inhibit the function of TaVIT2, as it was able to complement Δccc1 yeast (data not shown). Vhp1 was used as a vacuolar marker, and the absence of actin shows the purity of the vacuolar fraction.