Figure 7.

Endosperm-specific overexpression of TaVIT2 in barley. The TaVIT2-5DL gene from wheat under the control of the wheat HMW-GLU-1D-1 promoter (for full details, see Fig. 3A) was transformed into barley cv Golden Promise. Positive transformants were selected by hygromycin. A, Mature barley T1 grains of a control plant and two transgenic lines stained with Perls’ Prussian Blue for iron. B, Element analysis in white and whole-meal flours from a control and two HMW-TaVIT2-overexpressing barley plants. The values are means of two technical replicates ± sd.