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. 2017 Jul 26;73(Pt 8):450–454. doi: 10.1107/S2053230X17009566

Table 2. Crystallization.

Method Hanging-drop vapour-diffusion method
Plate type 24-well hanging-drop plate (Hampton Research)
Temperature (K) 289
Protein concentration (mg ml−1) 12
Buffer composition of protein solution 10 mM HEPES, 75 mM NaCl pH 7.2
Composition of reservoir solution 0.1 M bis-tris pH 5.5, 17% PEG 3350
Volume and ratio of drop 2 µl, 1:1
Volume of reservoir (µl) 1000