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. 2017 Jul 26;73(Pt 8):469–475. doi: 10.1107/S2053230X17010007

Table 1. Crystallization conditions.

Method Vapour diffusion, sitting-drop
Plate type 96-well
Temperature (K) 291
Protein concentration (mg ml−1) 20
Buffer composition of protein solution 10 mM Tris–HCl pH 8.0
Composition of reservoir solution 100 mM sodium acetate pH 5.5, 25%(w/v) PEG 3350, 5%(v/v) PEG 400, 3%(v/v) dioxane
Volume and ratio of drop 1 µl (1:1)
Volume of reservoir (µl) 80