Table 1. Crystallization conditions.
Method | Vapour diffusion, sitting-drop |
Plate type | 96-well |
Temperature (K) | 291 |
Protein concentration (mg ml−1) | 20 |
Buffer composition of protein solution | 10 mM Tris–HCl pH 8.0 |
Composition of reservoir solution | 100 mM sodium acetate pH 5.5, 25%(w/v) PEG 3350, 5%(v/v) PEG 400, 3%(v/v) dioxane |
Volume and ratio of drop | 1 µl (1:1) |
Volume of reservoir (µl) | 80 |