Skip to main content
. 2017 Jul 26;73(Pt 8):476–480. doi: 10.1107/S2053230X17010640

Table 3. Crystallization of AaL.

Method Sitting-drop vapor diffusion
Plate type 24-well plate
Temperature (K) 292
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 50 mM HEPES pH 8.00, 150 mM NaCl
Composition of reservoir solution 0.45 M ammonium chloride, 16% polyethylene glycol 3350
Volume and ratio of drop 1:1
Volume of reservoir (µl) 500