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. 2017 Jul 27;73(Pt 8):486–490. doi: 10.1107/S2053230X17009748

Table 2. Crystallization of C4.4A-D1D2.

Method Sitting-drop vapour diffusion
Plate type 24-well
Temperature (K) 293
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 20 mM Tris pH 7.5, 100 mM NaCl
Composition of reservoir solution 22.5%(w/v) PEG 4000, 0.1 M citric acid pH 3.6
Volume and ratio of drop 1:1
Volume of reservoir (ml) 1