Table 2. Crystallization conditions for latent wild-type MdPPO1 and the mutants MdPPO1-Ala239Thr, MdPPO1-Leu243Ala, MdPPO1-Glu234Ala and MdPPO1-Phe259Ala.

Method	Vapour diffusion (hanging drop)
Plate type	15-well EasyXtal plates (Qiagen)
Temperature (K)	293
Protein concentration (mg ml−1)	5–10
Buffer composition of protein solution	50 mM Tris–HCl pH 7.5, 200 mM NaCl
Composition of reservoir solution	50 mM Tris–HCl pH 7.0, 19–21% PEG 3350
Volume and ratio of drop	1 µl protein solution, 2 µl reservoir solution
Volume of reservoir (µl)	500