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. Author manuscript; available in PMC: 2017 Aug 4.
Published in final edited form as: Curr Protoc Plant Biol. 2016 Jun 10;1(2):293–306. doi: 10.1002/cppb.20014

Figure 2.

Figure 2

Key steps to prepare chromatin for sonication.

(A) Plant samples were cross-linked by 1% formaldehyde under vacuum. (B) After homogenization by grinding in liquid nitrogen, etiolated (left) and light-grown (right) samples were resuspended in Extraction Buffer I and filtered by cheesecloth and Miracloth. (C) Pellets after centrifugation in Extraction Buffer I. (D) Pellets after centrifugation in Extraction Buffer II. (E) Setup of sucrose gradient using Extraction Buffer III. (F) Pellets after centrifugation in Extraction Buffer III. (G) Pellets after centrifugation in nuclei lysis buffer.