Figure 2.

Relative vitellogenin expression messenger RNA (mRNA) of (A) Vg1 and (B) Vg2 in the ovary of Triatoma infestans adult females after injecting Vg1 double-stranded RNA (dsRNA) and Vg2 dsRNA, respectively. The transcript levels of Vg genes were measured by quantitative polymerase chain reaction (qPCR) using β-actin mRNA to normalize the expression levels. The error bars represent the standard deviation of the mean. Two asterisks on the standard error bar indicate significant difference between the mean of the treatment with dsRNA and the mean of the controls at P < 0.01. Control 1: group injected with nuclease-free water; Control 2: group no injected.