Figure 2.

Virus isolation from the serum sample of case B. (A) reverse transcriptase polymerase chain reaction detection of severe fever with thrombocytopenia syndrome virus S, M, and L segments in supernatants from the first to fourth passage. M, DNA ladder; P1-P4, supernatant from the first to fourth passage; N, negative control; (B) IFA detection of the viral protein expression of the isolated virus in infected cells. (a) immunofluorescences assay (IFA) detection of nucleoprotein expression in infected cells and (b) IFA detection of nonstructural proteins expression in infected cells.