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. Author manuscript; available in PMC: 2017 Aug 4.
Published in final edited form as: Methods Enzymol. 2015 Sep 6;569:63–78. doi: 10.1016/bs.mie.2015.08.011

Figure 2.

Figure 2

Purification schemes for SUN2-KASH complexes. (A) Purification of the SUN2-KASH3 complex is illustrated to exemplify the strategy used for SUN2 / MBP-KASH constructs. A representative gel filtration elution profile at each step is shown, and the proteins eluted under each peak are indicated. (B) Purification of the SUN2-KASH5 complex is illustrated to demonstrate the strategy used for SUN2 / GB1-KASH constructs.