Table 1.

Primers used for analysis of the expression of SIRT1-SIRT7 in human PBMC

	Primers	Exons
SIRT1	F: 5′ACAGGTTGCGGGAATCCAAAGG3′ R: 5′CCTAGGACATCGAGGAACTACCTG3′	E5 E7
SIRT2 a	F: 5′CCTCGCCTGCTCATCAACA3′ R: 5′TCCTCCGAGGCCCATAATC3′	E13 E14
SIRT3	F: 5′GCTGACGTGATGGCAGACA3′ R: 5′AACCACATGCAGCAAGAACCT3′	E5 E5
SIRT4	F: 5′ACAGGGTCCTGTGCTTGGATTG3′ R: 5′TTCAGGACTTGGAAACGCTCTTGC3′	E2/3 E3
SIRT5	F: 5′AAGGCTGGCACCAAGAAC3′ R: 5′GCCACAACTCCACAAGAG3′	E5 E6
SIRT6	F: 5′TGGTCTCCAGCTTAAACAG3′ R: 5′AAGGCAGTGCAAGCCTCT3′	E8 E8
SIRT7 a	F: 5′CGTCCGGAACGCCAAATAC3′ R: 5′GACGCTGCCGTGCTGATT3′	E3 E3/4
ACTB	F: 5′TTCTACAATGAGCTGCGTGTG3′ R: 5′CAGCCTGGATAGCAACGTACA3′	E3 E4

F: forward; PBMC: peripheral blood mononuclear cells; R: reverse.

^aPrimers as in Ashraf et al.³⁵